Autoria: CARNEIRO, M. C.; CASTRO, A. C. S. e; SILVA, R. R.; CHAVES, J. E. V.; SILVA, V. A. O.; CAPOBIANCO, N. E.; QUEIROZ, P. J. B.; MELO, L. de F. e; BARBOSA, E. A.; DODE, M. A. N.; SPRÍCIGO, J. F. W.

Resumo: This study investigated the motility and fertility of frozen-thawed semen from Pantaneiro bulls, comparing samples collected via ejaculation and directly from the epididymis. The aim was to evaluate the potential of epididymal semen for use in conservation programs by assessing its post-thaw motility and in vitro fertility. Semen from five bulls was analyzed for fresh quality, cell-free DNA (cfDNA) levels, and sperm performance after freezing. Results showed that epididymal semen had fewer defects and exhibited superior motility after thawing compared to ejaculated semen. Despite these differences, both semen sources were equally effective in fertilizing oocytes in vitro. The characterization of cfDNA levels adds valuable insights to the field of male fertility and supports efforts to conserve indigenous cattle breeds. This research contributes to improving semen preservation techniques and highlights the motility of epididymal semen as a reproductive resource in livestock breeding and conservation strategies.

Ano de publicação: 2025

Tipo de publicação: Artigo de periódico

Unidade: Embrapa Recursos Genéticos e Biotecnologia

Palavras-chave: IVP, Indigenous cattle, Molecular marker, Semen cryopreservation